Characterization of the Light-Harvesting Antennas of Photosynthetic Purple Bacteria by Stark Spectroscopy. 1. LH1 Antenna Complex and the B820 Subunit from Rhodospirillum rubrum
نویسندگان
چکیده
Lucas M. P. Beekman,† Martin Steffen,§ Ivo H. M. van Stokkum,† John D. Olsen,‡ C. Neil Hunter,‡ Steven G. Boxer,§ and Rienk van Grondelle*,† Department of Physics & Astronomy, Vrije UniVersiteit, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands; Krebs Institute for Biomolecular Research and Robert Hill Institute for Photosynthesis, Department of Molecular Biology and Biotechnology, UniVersity of Sheffield, Sheffield S0 2TN, U.K; and Department of Chemistry, Stanford UniVersity, Stanford, California 94305-5080
منابع مشابه
Oligomerization of light-harvesting I antenna peptides of Rhodospirillum rubrum.
We investigated the oligomerization of the core light-harvesting complex (LH1) of Rhodospirillum rubrum from the separated alpha beta BChl(2) subunits (B820) and the oligomerization of the B820 subunit from its monomeric peptides. The full LH1 complex was reversibly associated from B820 subunits by either varying the temperature in the range 277-300 K or by varying the detergent concentration i...
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An additional component in the purified core light-harvesting complex (LH1) from wild-type purple photosynthetic bacterium Rhodospirillum rubrum has been identified as an oxidized species of alpha-polypeptide by MALDI-TOF mass spectrometry. This component appears as a slightly earlier-eluting peak in the RP-HPLC chromatogram compared with the authentic alpha-polypeptide. The oxidation site has ...
متن کاملTemperature-Dependent Lifetimes and Quantum Yield of the Singlet and Triplet States of the B820 Subunit of LHI Antenna Complex of Purple Bacterium Rhodospirillum rubrum
The quantum yield and lifetimes of fluorescence and triplet state of the B820 subunit of core antenna of photosynthetic purple bacterium Rhodospirillum rubrum are reported at temperature range from 4.2 to 300K. The fluorescence quantum yield of B820 is strongly dependent on temperature. From 4.2 to about 200 K the yield remains nearly constant. A nonradiative decay process becomes activated aro...
متن کاملPolarization angle dependence of stark absorption spectra of spirilloxanthin bound to the reconstituted LH1 complexes using LH1-subunits isolated from the purple photosynthetic bacterium Rhodospirillum rubrum.
Reconstituted LH1 complexes were prepared using the LH1 subunit-type complexes, isolated from the purple photosynthetic bacterium Rhodospirillum (Rs.) rubrum, and purified all-trans spirilloxanthin. Stark absorption spectra of spirilloxanthin bound to both the native and reconstituted LH1 complexes were compared in different polarization angles (χ) against the external electric field. From the ...
متن کاملThree pulse echo peak shift measurements on the B820 subunit of LH1 of Rhodospirillum rubrum
Ž . Three pulse photon echo peak shift measurements 3PEPS of the detergent isolated B820 subunit of the light harvesting complex of Rhodospirillum rubrum are presented and compared with 3PEPS data for the LH1 complex of Rhodobacter sphaeroides. The comparison suggests that the 90 fs component of LH1 3PEPS is indeed the energy transfer component. The striking similarity between 3PEPS of LH1 and ...
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